cd68 ed1  (Bio-Rad)

Journal: bioRxiv

Article Title: Magnetic Resonance Imaging for Improved Brain Tumor Detection

doi: 10.1101/2025.05.06.652446

Figure Lengend Snippet: A timeline description of the methodology of this study. Rats were injected with the tumor cells (F98) and scanned three weeks later. pre- and post-Gd imaging was performed using the same MR sequences (T 1 , T 2 , CEST, NOE). The brains of these rats were extracted the same day for histological analysis (hematoxylin and ED1 staining).

Article Snippet: Each section was treated with a 1:500 dilution of Mouse-anti-rat ED1 (CD68) (BioRad # MCA341GA) overnight at 4°C in humid chamber.

Techniques: Injection, Imaging, Staining

Journal: bioRxiv

Article Title: Magnetic Resonance Imaging for Improved Brain Tumor Detection

doi: 10.1101/2025.05.06.652446

Figure Lengend Snippet: Immunohistochemical staining was performed using Hematoxylin and Anti-CD68 antibody [ED1] to visualize general tissue architecture and macrophage distribution, respectively. The hematoxylin-stained section shows the full brain slice corresponding to the tumor region from one representative rat, with zoomed-in views highlighting the tumor core, tumor boundary, and regions enriched with macrophages. ED1 staining confirms the presence of macrophages, particularly concentrated along the tumor periphery.

Article Snippet: Each section was treated with a 1:500 dilution of Mouse-anti-rat ED1 (CD68) (BioRad # MCA341GA) overnight at 4°C in humid chamber.

Techniques: Immunohistochemical staining, Staining, Slice Preparation